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No.109 Analysis of compounds containing phosphate groups I

Biological substances


Develosil UHPLC C30, 1.6um (2.0x50mm)

Application Data No.109

Technical Report No.24 「Analysis of Peptides with UHPLC」

Biological substances

We analyzed peptides using “Develosil UHPLC series” announced on Tuesday, October 23, 2018.


A major feature is that it is possible to change the analytical conditions that used ion-pairing reagents such as TFA until now to mild mobile phase such as ammonium formate.

Changing the pH from 3 to 7 makes it possible to correspond to LC / MS negative mode as well.


Technical Report No.24

No.108 Dicarboxylic acids



Develosil HSR AQ C18, 5um (4.6x150mm)

Application Data No.108

No.107 Glutathione (Re, Ox)

Biological substances


Develosil HSR C18 Peptide, 5um (4.6x150mm)

Develosil HSR C18 Peptide, 3um (4.6x75mm)

Application Data No.107

No.106 Amyloid β-Protein

Biological substances

Amyloid β-protein is a peptide of about 40 amino acids, and it is said that accumulation of this substance is closely related to Alzheimer’s disease.

We analyzed amyloid β-protein using a Corona detector this time. In addition, this method can also be used for LC / MS by changing the column inner diameter to 2.0 mm.

Application Data No.106

No.105 Digested Protein

Biological substances

It is an analysis example in which insulin, BSA, monoclonal antibody, thyroglobulin are digested using a convenient kit.

In order to obtain more information, 2um particle size may be used, but in this data it is an analysis example using 3um particle size and a general-purpose HPLC system, so it is easy to analyze digests.

Application Data No.105

No.104 PTH-Amino Acids


It is an analysis example of 20 amino acids derivatized by PTH (phenylthiohydatoin).

Combination of column + UHPLC system with particle size 3 um and length 250 mm can realize stable analysis and high accuracy analysis at all times without worrying about the pressure limit.

Application Data No.104

No.103 Oligo Deoxy Thymidine

Biological substances

This is an example of analysis of oligodeoxythymidine using HPLC and UHPLC system.

Simply by selecting the column size and particle size according to your system you can easily analyze oligonucleic acids.

This data is not data transferred method from HPLC to UHPLC.

Application Data No.103

No.102 Analysis of catechins in tea (UHPLC)


We analyzed 4 kinds of catechins and caffeine contained in tea.

The UHPLC condition transferred from HPLC method greatly reduces injection volume, solvent usage and analysis time.

In particular, a column with an inner diameter of 2.0 mm and a length of 50 mm reduces analysis time by 85% while maintaining sufficient separation to achieve high efficiency.

Application Data No.102

No.101 dNTP (4mixture) for UHPLC

Biological substances




Develosil HSR AQ C18, 5um (4.6x150mm)

Develosil HSR AQ C18, 2um (2.0x50mm)

Application Data No.101